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Established A Index Adapters Consult with Index 2 (i5) Orientation For more info on how to enter i5 bases about the sample sheet in ahead or reverse complement orientation.
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Check with Index 2 (i5) Orientation To find out more on how to enter i5 bases within the sample sheet in forward or reverse enhance orientation.
Established B Index Adapters Refer to Index 2 (i5) Orientation for more information regarding how to enter i5 bases on the sample sheet in ahead or reverse enhance orientation.
This permits substantial numbers of libraries to be pooled and sequenced concurrently in the course of a single operate. In comparison with other indexing techniques, one of a kind dual indexing mitigates index hopping by filtering hopped reads.
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When assessing index sets for shade balance, the sequence noticed from the instrument needs to be assessed, regardless of the orientation useful for the sample sheet. To the i7 index, always asses shade harmony utilizing Réseau secret the "i7 bases for sample sheet" column orientation.
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